Re: breast tissue fixation

Stephen Slap describes Hacker's offering of >>JFC solution , a proprietary, 
patented (sorry, John Kiernan) mixture of ethyl and isoproyl alcohols with a 
long-chain hydrocarbon which dehydrates and clears the tissue 
simultaneously.<< for processing fatty breast tissue.

The problem here isn't so much the trade-secret formula - we're learning to 
live with more and more of this - but the lack of documentation that the 
product will support immunochemical stains such as the hormone receptors and 
HER2/neu. The latter is a particularly serious problem, because of the 
rigidly defined protocols required to determine whether or not the antibody 
directed against HER2/neu is to be used therapeutically. The not always 
practical alternative would be to fix some of the tumor overnight in formalin 
and do the tests on that block.

Bob Richmond
Samurai Pathologist
Knoxville TN

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