Re: Woodchuck tissue in Millonigs fixative
|From:||Bonnie P Whitaker <Bonnie.P.Whitaker@uth.tmc.edu>|
We like Carson's (another modified Millonig) for anything that might get EM.
We also use it for kidneys from outside, then we decide what to send for EM
and what to process for light microscopy. You use it just like regular
formalin as far as processing, etc.
----- Original Message -----
From: Gayle Callis <email@example.com>
To: <GSennello@nexstar.com>; <firstname.lastname@example.org>
Sent: Monday, January 29, 2001 10:39 AM
Subject: Re: Woodchuck tissue in Millonigs fixative
> We had better luck with Trumps 4% gluteraldehyde/4% formaldehyde in
> Sorensons Phosphate buffer, pH 7 to 7.4. We processed this the same as
> paraffin method for animal tissues. Tissues stored in fixative were good
> for a year or so on shelf for EM. EM results were far better than with
> modified Millonigs buffered formalin. It can be shipped to you in
> fixative, stored at RT on shelf until used.
> You can process in paraffin up to 1 hour per station. For EM, tissue
> be minced, rinsed with buffer and processed into epoxy of choice.
> At 11:21 AM 1/25/01 -0700, you wrote:
> >I have been asked to process woodchuck tissue which I have never done
> >before. I am sure someone out there can help me, Thanks in advance. My
> >questions are:
> >1. They want to fix in Millonigs since there is a chance that EM might be
> >required at some point. The tissue is being collected at an outside
> >contractor so I need to tell how long to leave it in the Millonigs and to
> >what solution it should be transferred into before shipment to me.
> >2. Does anyone have a processing schedule they would be willing to share?
> >Gina Sennello
> >Gilead Sciences
> >2860 wilderness Place
> >Boulder, CO 80302
> >Fax 303-444-0672
> >Phone 303-546-7739
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
> 406 994-6367
> 404 994-4303 (FAX)
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