Re: Wenger-Angritt Helicobacter stain
Dr David A. Agbamu at the Arrowe Park Hospital, UK suggested (on the PATHO-L
list) looking at:
Histological identification of Helicobacter pylori: comparison of staining
methods. O Rotimi, A Cairns, S Gray, P Moayyedi, M F Dixon (Department of
Histopathology, The General Infirmary at Leeds UK). J Clin Pathol
2000;53:756-759
available as a PDF file from
http://jcp.bmjjournals.com/cgi/reprint/53/10/756.pdf
These authors examined about 60 well documented cases, half of them positive
for Helicobacter pylori and half of them negative, using a modified Giemsa,
an immunohistochemical technique, a silver stain, and a Gimenez carbol
fuchsin stain. All of the stains worked well, but the modified Giemsa was a
great deal simpler to do, and they preferred it for that reason. They did not
look at the effectiveness of the H and E stain by itself as a stain for
Helicobacter.
I'm glad to know that the Journal of Clinical Pathology is available online!
This excellent journal is not easily available in libraries in the USA.
Bob Richmond
Samurai Pathologist
Knoxville TN
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