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<font size=3>Bob,<br>
<br>
I have done Giemsa, Giemenez,Diff-Quik, Warthin-Starry, And
Steiner for h.pylori. Recently I tried a Cresyl Echt Violet. The bugs are
more easily seen in a well stained silver. Both the W-S and steiner
are short microwave methods. The problem with the silvers ,an occasional
precip.<br>
<br>
Rena Fail<br>
<br>
<br>
<br>
<br>
<br>
At 09:44 PM 1/24/01 -0500, you wrote:<br>
<blockquote type=cite class=cite cite>Last October Sue Barnes
(SBarnes@elch.org) posted: >>I am looking for the <br>
Wenger-Angritt stain for Helicobacter pylori. This stain was mentioned in
an <br>
AFIP report and my pathologist would like to start doing
it.<<<br>
<br>
Apparently a recent pathology book - perhaps an AFIP fascicle on GI
pathology <br>
- refers to this stain, apparently in use at the AFIP, and one of my
locum <br>
tenens clients asked for it. The laboratory contacted Dr. Helen E.
Remotti at <br>
AFIP yesterday, and she faxed a procedure. This next paragraph is mostly
<br>
copied from that procedure:<br>
<br>
>>This modification of Manuel's reticulum stain demonstrates some
<br>
argyrophilic microorganisms. It is a synthesis, with modifications, of
<br>
several silver impregnation methods, notably Gomori's original method and
<br>
Manuel's reticulum procedure as found in the AFIP manual. The stain is
done <br>
on paraffin sections of formalin fixed tissues. It uses the <br>
permanganate-metabisulfite sequence to clear the background, followed by
<br>
uranium nitrate sensitization and modified Fontana's ammoniacal silver
for <br>
impregnation. The wash following the silver impregnation contains the
<br>
reducing agent (formalin) in order to minimize the loss of silver.
Sections <br>
are checked microscopically after the reduction step. No counterstain is
<br>
used. The treatments with gold chloride and sodium thiosulfate are
omitted to <br>
optimize bacterial staining.<<<br>
<br>
Besides the 1968 AFIP manual, the only reference is to the AFIP Letter,
Vol <br>
155, No 1, page 8, February 1997.<br>
<br>
Obviously this isn't a real-world procedure - it's time consuming, few
<br>
clinical labs can differentiate sections under a microscope, and the
uranyl <br>
nitrate would have the Herrn Inspektors at your door in short order. -
Once <br>
again I ask - has this, or any silver impregnation procedure, been shown
to <br>
be more sensitive in finding Helicobacter pylori?<br>
<br>
Bob Richmond<br>
Samurai Pathologist<br>
Knoxville TN</font></blockquote><br>
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