Re: Urgent question

From:Anita Jennings <>

Get in touch with your Dako rep and try their anti-rat that is absorbed
against mouse. I use their anti-mouse on mouse tissue and it is very clean.
I believe the number I use is F313. It is worth a try anita

> From: Lynn Gardner <>
> Date: Thu, 25 Jan 2001 19:17:17 -0600
> To:
> Subject: Urgent question
> Dear Friends,
> We are staining for anitbodies in nuede mice. They have been injected with
> rat cells and we want to demonstrate the antibody in the rat cells. The
> antibody is a rabbit anti-rat antibody. The problem is we get staining in
> the tissue but everything is staining. The more worrysome problem is that
> the Rabbit IgG negative control is also staining vibrantly positive even
> more so than the antibody stained tissue. Does this mean that the rabbit
> anti-rat antibody and the Rabbit IgG are cross reacting with the mouse
> tissue? or something else?
> I block for Hydrogen peroxide, avidin and biotin and use a protein block
> that is serum free. I can't figure this one out. Any hints would be most
> appreciated!!!
> Thanks gang,
> Lynn 

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