Re: Trichrome for Fresh Frozen sections

From:Rena Fail <>

<html> <font size=3>Jen,<br> <br>     I use a Gomori's one-step to stain fresh frozen muscle. No Mordant.1 minute in filtered Harris Hematoxylin, 5 minutes washed in tap water, 10 minutes in filtered Gomori's one step, Rinse quickly in 0.2%<br> Acetic Acid, dehydrate,clear and mount.<br> <br> Rena Fail AS,HT(ASCP)<br> Medical University <br> Charleston, SC<br> <br> 05:41 PM 1/19/01 -0500, you wrote:<br> <blockquote type=cite class=cite cite>Hi.<br> Thank you to all who responded to my problem with skin processing.  The<br> insight was so helpful.<br> <br> Now do any of you have a procedure to stain fresh frozen sections with<br> Masson's Trichrome?  We're not sure how to fix the tissue prior to staining.<br> With FFPE tissue we de-paraffin, re-hydrate, and then plunk into warm Bouins<br> for 60 minutes.  I was thinking we could just go right into Bouin's with the<br> frozen sections.<br> <br> Thanks in advance.<br> <br> Jen<br> <br> <br> <br> Jennifer M. Philopena<br> Scientist 1<br> Canji, Inc.<br> 3525 John Hopkins Court<br> San Diego, CA 92121<br></font></blockquote><br> </html>
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