Sigma has a good kit (Leukocyte acid phosphatase, Cat# 387 A) that works
well on mouse feet.

Gina Sennello
Gilead Sciences
Boulder Co




                                                                                         
                    "Ford, Judi                                                          
                    {Path~Palo           To:     "Histonet (E-mail)"                     
                    Alto}"               <histonet@pathology.swmed.edu>                  
                    <JUDI.FORD@RO        cc:                                             
                    CHE.COM>             Subject:     TRAP Staining Method               
                                                                                         
                    01/18/01                                                             
                    05:17 PM                                                             
                                                                                         
                                                                                         



Hi everyone:

I want to thank all those who gave me input on my search for tungsten
knives.  My supervisor
was pleased with the response.  I have another need at the moment.  One of
our investigators
is interested in doing the TRAP stain on mouse hind feet.  Does anyone have
a reference for
the method, or the method itself?  Has anyone done it on mouse feet?  I'd
be
grateful for any
info sent my way.

Thanks!

Judi Ford
Roche
Palo Alto, CA