Re: Slide adhesives

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>



Helen Wimer wrote:

> >>> Geoff McAuliffe <mcauliff@UMDNJ.EDU> 01/31/01 17:34 PM >>>
> Darren Robinson wrote:
>
> > Does anybody out there have an idea as to how I can get large
> > methylmethacrylate sections (3"x3") to stick onto glass slides.  I am
> > cutting some really nice large sections, getting them onto the slides, then
> > losing them when I either try and dissolve the resin in xylene or stain
> > them with heamalum.  I've tried APES, and sta-on, and am running out of
> > ideas fast.
> >
> > Many thanks,
> >
> > Darren
> >
> > ****************************************
> > Darren Robinson
> > Postdoctoral Fellow
> > CIIT Centers for Health Research
> > 6 Davis drive
> > Research Triangle Park
> > North Carolina
> > 27709
> >
> > Tel. 919-5581385
> > Email. DRobinson@ciit.org
> > ****************************************
>
>     Hot melt glue. Yes, I am serious. Years ago there was a short paper in
> "Stain Technology" (now known as Biotechnique and Histochemistry) about using
> hot melt glue at the corners of large plastic sections to hold them down. I
> think they were sectioning lumbar vertebrae or some other big thing. If I can
> find the exact reference I will pass it on to you.
>     So go to Home Depot and get a hot melt glue gun and some glue sticks.
>
> Geoff
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
> I am curious ...did the hot melt glue leave a backround stain on the slide?  Thanks.  Helen

    I really don't remember. The author used relatively small spots of glue at the corners of
the sections.
    Hey, can the sections be stained free-floating and then mounted on slides?

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************





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