Re: Shelf life of Saf-O

From:"J. A. Kiernan" <>

On Mon, 22 Jan 2001, Jerry Chi-Yuan Hu wrote:

> I just mixed myself two aqueous solutions of 0.1% Safranin O and 0.05%
> Fast Green.  I keep them in amber glass bottles, away from the light,
> since I suspect that they can photobleach.  I was wondering if anybody
> knows how long these stains can keep this way - I did not see an
> expiratory date on the bottles that the dry dyes came in.  

Churukian's "Manual" says 6 months for solutions of both dyes.
I think this may be rather conservative, having kept fast green FCF
solutions for several years without apparent deterioration. 
I don't use safranine as much, but 2 year-old solutions don't seem
to me to behave any differently from new ones.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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