Re: Shelf life of Saf-O
|From:||"J. A. Kiernan" <email@example.com>|
On Mon, 22 Jan 2001, Jerry Chi-Yuan Hu wrote:
> I just mixed myself two aqueous solutions of 0.1% Safranin O and 0.05%
> Fast Green. I keep them in amber glass bottles, away from the light,
> since I suspect that they can photobleach. I was wondering if anybody
> knows how long these stains can keep this way - I did not see an
> expiratory date on the bottles that the dry dyes came in.
Churukian's "Manual" says 6 months for solutions of both dyes.
I think this may be rather conservative, having kept fast green FCF
solutions for several years without apparent deterioration.
I don't use safranine as much, but 2 year-old solutions don't seem
to me to behave any differently from new ones.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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