Re: Negative control on IHC's
The way it was explained to me, is that it is possible for each individual
block to show a false positive, therefore, a negative is ran with that
species type antibody on each block used to verify that if there is positive
staining, that it in fact is a true positive staining. Also, it was
explained to me that if you are running a variety of stains, and some are
mono-clonal (mouse) and some poly-clonal (rabbit), you will need to run a
negative mouse and rabbit on that one block. The reason being, if you ran
all of the stains and just one negative, say a mouse, the mouse negative may
show that there is no false positive staining when in fact if a negative
rabbit had been ran, there very well could have been false positive staining
in a certain area, therefore the poly-clonal antibody may have shown to be
positive, when in fact, it was a false positive that went un-detected because
there was not a negative rabbit ran with that case. That is the reason for
running the negative slides on each block. I hope that I did not go on too
much.
Jeanie Wade, H. T. (ASCP)
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