Re: Kappa & Lambda

From:Amos & Theresa <>

    Try no antigen retrieval at all. The citrate will really give you a
lot of background staining. Ours is at 60 K and 30 K, respectively, with
acceptable results. Also Envision+ helps get rid of any endogenous
good luck
Amos Brooks

Dana Settembre wrote:

> Need help in reducing Kappa & Lambda background on bone marrow.
> I use Dako's antibody, Kappa at 15K and Lambda at 18K, tonsil as a
> control, citra MW 15 minutes for pretreatment.
> Any suggestions?
> Dana Settembre
> Immunohistochemistry Lab
> Pathology Department
> University Hospital
> Newark,  New Jersey

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