Re: Hif1a immuno
|From:||Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>|
Try long HEIR, 45 min pH6.0 citrate (ya 45 minutes!) on brain or colon l.
1:800 overnight staining at 4C, with with Dako CSA (tyramide
amplification). Honestly, i think this is excessive for any antibody,
but that is the protocol directly obtained from one of the researchers who
developed the marker.
Richard Lea wrote:
> Dear Histonetters,
> Is anyone looking at Hif1alpha by immunohistochemistry in wax sections.
> If so can you advise on the protocol i.e., antigen retrieval etc. We
> are using rat placenta and tried to use liver as a positive control
> tissue. The placenta came up but very little in the liver - any
> suggestions ?
> Many thanks,
> Richard Lea
> Richard G. Lea PhD
> Sr. Research Scientist, Division of Nutrition and Development
> Hon. Lecturer, Dept. Obstetrics & Gynaecology, University of Aberdeen
> Rowett Research Institute
> Greenburn Road
> Aberdeen AB21 9SB
> Tel: 44(0) 1224 712751 x 2319
> Fax: 44(0) 1224 716622
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