Try long HEIR,  45 min pH6.0 citrate (ya 45 minutes!) on brain or colon l.
1:800 overnight staining at 4C, with with Dako CSA (tyramide
amplification).  Honestly,  i think this is excessive for any antibody,
but that is the protocol directly obtained from one of the researchers who
developed the marker.
Good Luck
Luis

Richard Lea wrote:

> Dear Histonetters,
>
> Is anyone looking at Hif1alpha by immunohistochemistry in wax sections.
> If so can you advise on the protocol i.e., antigen retrieval etc.  We
> are using rat placenta and tried to use liver as a positive control
> tissue.  The placenta came up but very little in the liver - any
> suggestions ?
>
> Many thanks,
>
> Richard Lea
>
> --
> Richard G. Lea PhD
> Sr. Research Scientist, Division of Nutrition and Development
> Hon. Lecturer, Dept. Obstetrics & Gynaecology, University of Aberdeen
> Rowett Research Institute
> Greenburn Road
> Bucksburn
> Aberdeen AB21 9SB
>
> Tel:  44(0) 1224 712751 x 2319
> Fax:  44(0) 1224 716622