Re: DAB and bleach
From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
On Mon, 22 Jan 2001, Cathy Gorrie wrote:
> I still use bleach!!! I hadn't heard it still leaves DAB mutagenic!!!
So does nearly everyone else, but acid permanganate brings about
a more complete oxidation and is the approved method in textbooks
of lab safety. It's a matter of complying with laws and other
regulations more than one of common sense, unfortunately.
There was much correspondence about this on Histonet about
2 years ago, which should be recorded in the archives at:
www.histosearch.com. Some of this is abstracted in an FAQ
at: publish.uwo.ca/~jkiernan/faqlist.htm#MISCE
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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