Re: Cutting fixed brains at 20 microns

From:"Markus F. Meyenhofer" <>

Nancy Maronto wrote:
> We are asked to cut 20 micron sections of rat brains perfused in a
> paraformaldehyde fixative.  Can you experts out there suggest ways of doing
> this.  I know of adding sucrose to tissue and doing frozen sections. Which
> may be very difficult.  The equipment we have is a tissue tech cryostat and
> rotary microtomes.  Thanks in advance for your help.
> Nancy Maronto
> MPI Research

A Vibratome will cut 20u + sections with ease.
We have a few reconditioned units available. Please contact me direct.
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
fax 732 758 9142
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