Re: Cutting fixed brains at 20 microns (Flores, Teresa)

Nancy, There must be a new publication out,as our histology lab was
recently asked to do this also. Fortuneately we were able to direct the
investigator to a sliding microtome that is being used in our muscle lab.
Good luck! Teresa
 >We are asked to cut 20 micron sections of rat brains perfused in a
>paraformaldehyde fixative.  Can you experts out there suggest ways of doing
>this.  I know of adding sucrose to tissue and doing frozen sections. Which
>may be very difficult.  The equipment we have is a tissue tech cryostat and
>rotary microtomes.  Thanks in advance for your help.
>Nancy Maronto
>MPI Research
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