Re: Cutting fixed brains at 20 microns
|From:||firstname.lastname@example.org (Flores, Teresa)|
Nancy, There must be a new publication out,as our histology lab was
recently asked to do this also. Fortuneately we were able to direct the
investigator to a sliding microtome that is being used in our muscle lab.
Good luck! Teresa
>We are asked to cut 20 micron sections of rat brains perfused in a
>paraformaldehyde fixative. Can you experts out there suggest ways of doing
>this. I know of adding sucrose to tissue and doing frozen sections. Which
>may be very difficult. The equipment we have is a tissue tech cryostat and
>rotary microtomes. Thanks in advance for your help.
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