Re: CD4 1F6

Hello Tim,

we are using the 1F6 from Novocastra on formalin fixed cynomolgus (macaque)
tissue, especially in spleen. After microwaving in 1mM EDTA, pH 8.0 for 15
min and a dilution of 1:40 it works nicely for us. In contrast to the
datasheet we were able to use the antibody also successfully for frozen
tissue. For the formalin fixed tissue I need to say that it is generally
fixed more than 48 hrs.
Just try it again !
Good luck,

Antje Marcantonio
Novartis Pharma AG

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