Re: CD3 for Veterinary Medicine

From:Gayle Callis <uvsgc@montana.edu>

Maybe I missed it, but what species are you working with, dog, cat, horse,
bovine, etc???? for this CD3 staining?



At 09:18 AM 1/30/01 -0600, you wrote:
>Louri,
>
>I use the polyclonal CD3 from DAKO; microwave pretreatment in citrate
>buffer - 2 x 5 minutes with 20 minute cooldown; H2O2 block for 15 minutes;
>normal goat serum protein block for 15 minutes prior to primary antibody;
>CD3 at 1:50 for 45 minutes, LSAB2 link and label for 15 minutes each; AEC
>for 10 minutes.  All steps from H2O2 block on - done on autostainer.  I make
>my CD3 dilution from concentrate right before each time I use it.
>
>Jan Shivers
>U of MN Vet Diag Lab
>  ----- Original Message -----
>  From: Louri Caldwell
>  To: histonet@pathology.swmed.edu
>  Sent: Monday, January 29, 2001 7:52 PM
>  Subject: CD3 for Veterinary Medicine
>
>
>  Hello everyone,
>
>  We currently are having inconsistencies with our CD3 marker.  I was
>wondering if  any of you may be using an antibody that is providing good
>results?  I would like to try another source, and any suggestions you may
>have would be greatly appreciated.
>
>  Thank you in advance for your help.
>
>  Louri Caldwell
>
>
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Gayle Callis
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)




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