Louri,

I use the polyclonal CD3 from DAKO; microwave pretreatment in citrate
buffer - 2 x 5 minutes with 20 minute cooldown; H2O2 block for 15 minutes;
normal goat serum protein block for 15 minutes prior to primary antibody;
CD3 at 1:50 for 45 minutes, LSAB2 link and label for 15 minutes each; AEC
for 10 minutes.  All steps from H2O2 block on - done on autostainer.  I make
my CD3 dilution from concentrate right before each time I use it.

Jan Shivers
U of MN Vet Diag Lab
  ----- Original Message -----
  From: Louri Caldwell
  To: histonet@pathology.swmed.edu
  Sent: Monday, January 29, 2001 7:52 PM
  Subject: CD3 for Veterinary Medicine


  Hello everyone,

  We currently are having inconsistencies with our CD3 marker.  I was
wondering if  any of you may be using an antibody that is providing good
results?  I would like to try another source, and any suggestions you may
have would be greatly appreciated.

  Thank you in advance for your help.

  Louri Caldwell


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