|From:||Patti Loykasek <firstname.lastname@example.org>|
on 1/31/01 11:02 AM, Dana Settembre at settembr@UMDNJ.EDU wrote:
> On Mon, 29 Jan 2001, Terrett, Barb wrote:
>> Is anyone using CD25 (ours is from Novocastra) , and if so how is it working
>> for you? We have had some problems (using it on FFPE) . We are microwaving
>> it in citrate buffer pH 6.0 and staining with the envision kit. I was
>> gettingtoo much background with my usual link and label system, even at a
>> high titre.
>> Thanks for your help
> Hello Barb,
> I too was having problems with CD25 but got it to work as follows:
> pretreat with Dako's TRS (not the high pH one)
> 1:50 dilution and 60 minute incubation using a tonsil as positive control.
> Good Luck.
> Dana Settembre
> Immunohistochemistry Lab
> Pathology Department
> University Hospital
> Newark, New Jersey
You might want to try a 40 minute steam antigen retrieval in EDTA, and a
titer of 1:100. Good Luck.
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