Hi,
    We are getting good staining at 1:200 with no pretreatment at all. It was
stubborn though at first, it needs Envision + or some other non biotin
secondary. The background is probably biotin.
Amos Brooks

Teri Johnson wrote:

> Hi out there in histoland!  Is anyone using CD117, polyclonal antibody?  We
> are titering it and have noticed some non-specific staining in muscle. The
> spec sheet says to avoid proteolytic enzymes (so we avoided them).  It also
> indicates that the antibody can be used "without the antigen retrieval,
> while pretreatment by heating sometimes gives better staining."  What in the
> heck is that supposed to mean?
>
> -Teri Johnson
> Histology Supervisor
> Physicians Reference Laboratory
> Overland Park, KS