RE: flotation of bone(tissue) on slides

From:"Su, Phy-Huynh" <>

I've found that it worked better for me if I fixed the tissues well first,
then decal.  It'll take longer time, but I had better sections.  I also used
home-made silane slides.  The commercial "+" slides don't work well for
cartialge and bone tissues here.
> -----Original Message-----
> From: []
> Sent:	Monday, January 22, 2001 12:31 PM
> To:
> Subject:	flotation of bone(tissue) on slides
> Hi Histonetters,
> I need some advice on decalcification of mouse leg (1cm x1mm).  I let the 
> tissue fix in a decalcifier called "Cal-Rite" by Richard Allan Scientific
> for 
> over the weekend.  Cal-Rite is a fixative and decalifier in one. The bone
> felt 
> like rubber.  I then cut the leg into several little pieces(1 mm x 1 mm).
> When 
> I cut the tissue on the microtome(paraffin-embedded), it was very hard .
> So I 
> surface decal. for 30 minutes.  I went to go cut, it was fine.  But
> several 
> slides down the line, I had to surface again and again.  My slides came
> out 
> really bad.  The tissue was floating everywhere.  I used gelatin in my
> water 
> bath and superfrost slides(Fisher).  Would it make a difference if I 
> use "superfrost +" plus slides?  
> Any help would be greatly appreciated.
> Thanks for your time,
> Dina D. Gaupp, MT
> Tulane Medical School
> Center for Gene Therapy
> email:

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