RE: flotation of bone(tissue) on slides
|From:||"Su, Phy-Huynh" <email@example.com>|
I've found that it worked better for me if I fixed the tissues well first,
then decal. It'll take longer time, but I had better sections. I also used
home-made silane slides. The commercial "+" slides don't work well for
cartialge and bone tissues here.
> -----Original Message-----
> From: firstname.lastname@example.org [SMTP:email@example.com]
> Sent: Monday, January 22, 2001 12:31 PM
> To: firstname.lastname@example.org
> Subject: flotation of bone(tissue) on slides
> Hi Histonetters,
> I need some advice on decalcification of mouse leg (1cm x1mm). I let the
> tissue fix in a decalcifier called "Cal-Rite" by Richard Allan Scientific
> over the weekend. Cal-Rite is a fixative and decalifier in one. The bone
> like rubber. I then cut the leg into several little pieces(1 mm x 1 mm).
> I cut the tissue on the microtome(paraffin-embedded), it was very hard .
> So I
> surface decal. for 30 minutes. I went to go cut, it was fine. But
> slides down the line, I had to surface again and again. My slides came
> really bad. The tissue was floating everywhere. I used gelatin in my
> bath and superfrost slides(Fisher). Would it make a difference if I
> use "superfrost +" plus slides?
> Any help would be greatly appreciated.
> Thanks for your time,
> Dina D. Gaupp, MT
> Tulane Medical School
> Center for Gene Therapy
> email: email@example.com
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