You could make a cell block with the frozen scrapings. We make controls by
mixing bacteria cultures with finely-minced normal tissue and then
processing that into a paraffin block.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
Ms-G32
1600 Clifton Road
Atlanta, GA 30333
USA

PH: 404-639-3964
FAX: 404-639-3043

email: tim9@cdc.gov



-----Original Message-----
From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu]
Sent: Friday, January 26, 2001 9:55 AM
To: Histonet@pathology.swmed.edu
Subject: bacteria samples


Dear Histonetters,

I have been asked to ask if anyone has had experience immunostaining
bacteria samples from mucosa scrapings.  The scrapings have been stored in
tubes in a -70C freezer. Some concerns I have would be how to make slides
from the scrapings and how to get the scrapings to adhere well.

Elaine Dooley HTL
352-265-0111 ext 44935