We do protein electrophoresis and westerns on bone cells. I can share with
you what we do. What specifically are you interested in extracting? Marrow
cells (proteins) or Matrix proteins? The cell lysis solutions are quite
different. Glad to help any way we can. Regards, Donna Montague, UAMS
Physiology & Orthopaedic Research, Little Rock. AR

		-----Original Message-----
		From:	Todd Miller [mailto:tmiller103@hotmail.com]
		Sent:	Wednesday, January 31, 2001 9:05 AM
		To:	histonet@pathology.swmed.edu
		Subject:	Westerns for Bone

		Hi all.  Does anyone have a protocol for doing Westerns on
bone?  I suppose 
		I'll just need to know a way to extract the proteins, as
we've not yet 
		decided for sure what proteins we'll be measuring.  I just
wanted to know if 
		anyone has done this and how difficult it is.  Thanks.


		Todd
	
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