It's good practice to run a negative control on each block to test for
non-specific staining. If you are running more than one antibody from the
same animal species you can use each as a negative control for the other.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
Ms-G32
1600 Clifton Road
Atlanta, GA 30333
USA

PH: 404-639-3964
FAX: 404-639-3043

email: tim9@cdc.gov



-----Original Message-----
From: Laurie Colbert [mailto:laurie.colbert@schs.com]
Sent: Saturday, January 20, 2001 12:37 PM
To: Histonet
Subject: Negative control on IHC's


Is there a good reason to run a negative control on every block when
staining IHC's?  We do a lot of keratin stains on lymph nodes from the
same specimen (not part A, part B, etc.) and we run a negative control
along with the keratin on every block.

Laurie Colbert