We have used FITC labelled probes for detection of low level expression. A
good fluorescent scope or a Confocal scope aid in the detection. See info at
Molecular Probes. They have an extensive and helpful website. Good luck,
Donna Montague, Orthopaedic Research, UAMS, Little Rock, AR


-----Original Message-----
From: J. Hohmann [mailto:hohm@u.washington.edu]
Sent: Thursday, January 25, 2001 12:32 AM
To: HistoNet Server
Subject: Low abundance mRNA messages


I am looking for advice in non-radioactive detection of very low abundance
mRNA species in mouse brain. Standard biotin or dig-labeled protocols do
not work, and for logistical reasons we do not want to use isotopes
(although P-33 does detect these mRNAs). I am thinking of trying either
a tyramide amplification strategy, or perhaps an enzyme-linked flourescent
approach. Or maybe both together. Any help in this area would be Much
appreciated!

Thanks,
John Hohmann



*************************
 John Hohmann
 Neurobiology and Behavior Program
 Box 356460 OB/GYN
 University of Washington
 Seattle, Wa. 98195
 hohm@u.washington.edu
 PH # 206-543-9970
 FAX # 206-543-3915
*************************