If you require only small - a few mm - sections, than using a Vibratome is the 
simple way to do that. The tissue requires in fact no preparation, not even 
fixation.
Disclaimer: ProSciTech is a distributor of Vibratomes, though we would not sell 
into the US market.
Cheers
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560                      www.proscitech.com

On Tuesday, January 30, 2001 12:00 AM, Nancy Maronto 
[SMTP:nmaronto@hotmail.com] wrote:
> We are asked to cut 20 micron sections of rat brains perfused in a
> paraformaldehyde fixative.  Can you experts out there suggest ways of doing
> this.  I know of adding sucrose to tissue and doing frozen sections. Which
> may be very difficult.  The equipment we have is a tissue tech cryostat and
> rotary microtomes.  Thanks in advance for your help.
>
> Nancy Maronto
> MPI Research
>
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