RE: Breast Tissue Fixation

From:"Tom T. McNemar" <>

We are currently using CBA formalin.  We use it in the 2 formalin stations
on a backup processor.  We have begun to use it for overnight fixation as
well with better results (it doesn't cut any better but the pathologists
think the sections look better).  It is still pretty dependent on the size
of the tissue though.

Tom Mc Nemar, HT(ASCP)
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	Steven E. Slap []
> Sent:	Saturday, January 27, 2001 3:27 PM
> To:	HistoNet Server
> Subject:	Re: Breast Tissue Fixation
> Hi HistoNetters!
> Debra Ritter asked:
> "Would like to know if anyone out there uses a type of pre-fixative for
> fatty breast tissue before putting it in formalin on the processor?
> Sometimes our breast tissues even though they have been in formalin all
> week-end on the processor still cut lousy on Monday morn. Any info would
> be appreciated!"
> Processing breast tissue is one of the most pervasive problems in
> histology.  One solution is the use of microwave processing with JFC
> solution.  JFC solution is a proprietary, patented (sorry, John Kiernan)
> mixture of ethyl and isoproyl alcohols with a long-chain hydrocarbon which
> dehydrates and clears the tissue simultaneously.
> In microwave processing, after the tissue is exposed to JFC, it is put in
> paraffin and the alcohols are flash-evaporated.  Some residual hydrocarbon
> remains in the paraffin and makes the resulting blocks very easy to cut (I
> have heard them described as "buttery").
> JFC solution was developed by Milestone and is available in the United
> States through Hacker Instruments, my employer.
> Best regards,
> Steven Slap
> ************************************************
> Steven E. Slap
> Marketing Manager & Microwave Product Specialist
> Hacker Industries & Instruments
> 413-733-3808
> ************************************************

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