Microwave fixation of brains
|From:||Cheryl Crowder <email@example.com>|
Rosalba, Human (or other brains) can be fixed with microwaves but it
must be done in several steps. The brain is so large if you microwaved
it until it was totally fixed, parts would be overheated. Therefore,
what we have done is placed the brain in fixative to cover, microwave
until the solution reaches 55#176#C. Let the brain "rest" in the heated
solution for several minutes. The brain will then be firm enough to
slice without distortion.
The slices now produced are fixed in fresh solution to 55#176#C.
Although this seems to take a long time, it is really short - no more
than 30 minutes to get perfect gross slices.
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA 70803
FAX (225) 578-9720
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