Low abundance mRNA messages

From:"J. Hohmann" <hohm@u.washington.edu>

I am looking for advice in non-radioactive detection of very low abundance
mRNA species in mouse brain. Standard biotin or dig-labeled protocols do
not work, and for logistical reasons we do not want to use isotopes
(although P-33 does detect these mRNAs). I am thinking of trying either
a tyramide amplification strategy, or perhaps an enzyme-linked flourescent
approach. Or maybe both together. Any help in this area would be Much

John Hohmann

 John Hohmann
 Neurobiology and Behavior Program
 Box 356460 OB/GYN
 University of Washington
 Seattle, Wa. 98195
 PH # 206-543-9970
 FAX # 206-543-3915

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