Hello all,

Is there a way to prepare for sectioning cell monolayers which express GFP
without losing the fluorescence?  We want to do confocal microscopy on
semi-thin sections. We could do paraffin, frozen, or any type of resin
embedded samples.

Any helpful hints out there?

Thanks!

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren@umich.edu