"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Sue,<br>
<x-tab> </x-tab>My first
reaction is to fix and process for EM. Cut 1-2 micron sections and stain
with a suitable polychrome for epoxy resin.<br>
Ian.<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Thu, 25 Jan 2001 11:17:15
-0500<br>
From: STYLER@dnr.state.md.us<br>
Subject: embedding small critters<br>
To: histonet@pathology.swmed.edu<br>
<br>
<br>
Dear Histonetters:<br>
<br>
Could you give me advice on a "small" problem? I am working
with an animal<br>
that is 244 microns in size. We currently take digital photos to
identify<br>
the animal prior to embedding. The animal is then marked with eosin
for<br>
better viewing, transported by glass pipette to a piece of lenspaper
and<br>
covered with a drop of 1.7% agar for easier manipulation. The animal is
then<br>
processed on a 2 hour schedule and routinely embedded.<br>
<br>
In some cases, while embedding, the agar adhered to the lens paper. In
other<br>
cases, the animal was no longer present/visible but the agar plug
still<br>
remained. I sectioned and stained the remaining agar plugs to confirm
the<br>
presence or absence of the animal.<br>
<br>
<br>
I tried different concentrations of agar.<br>
The mesh in the histology body bags is to small.<br>
Growth hormone is not an option:)<br>
Can anyone offer processing suggestions?<br>
<br>
Sue Tyler HT (ASCP)<br>
Center for Coastal Environmental Health <br>
and Biomolecular Research<br>
Cooperative Oxford Lab<br>
904 South Morris Street<br>
Oxford, MD 21654<br>
styler@dnr.state.md.us <br>
<br>
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<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>