"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Jen,<br>
<x-tab> </x-tab>Muscle
histochemistry, the only sure way to avoid artifact is freeze in
iso-pentane cooled with liquid nitrogen. Cryoprotection is not
necessary.<br>
Ian.<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Wed, 24 Jan 2001 17:23:59
-0500<br>
From: "Philopena, Jennifer"
<jennifer.philopena@canji.com><br>
Subject: cryo-preservation<br>
To: "'Histonet'" <histonet@pathology.swmed.edu><br>
<br>
Happy Chinese New Year to all in Histoland.<br>
<br>
I am doing fresh frozen sections of muscle and am getting freezing
artifact.<br>
<br>
My protocol is to remove the muscle and embed in OCT in a CO2/
methylbutane<br>
slurry. Then I section at -18, fix the sections, and b-Gal
stain. My<br>
question is this: can I cryo-protect with sucrose prior to<br>
embedding/freezing without fixing the muscle first? Would the
sucrose<br>
interfere with staining?<br>
<br>
Thanks (again and again and again).<br>
Jen<br>
<br>
<br>
<br>
Jennifer M. Philopena<br>
Scientist 1<br>
Canji, Inc.<br>
3525 John Hopkins Court<br>
San Diego, CA 92121<br>
jennifer.philopena@canji.com</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>