Fwd: cryo-preservation

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> Jen,<br> <x-tab>        </x-tab>Muscle histochemistry, the only sure way to avoid artifact is freeze in iso-pentane cooled with liquid nitrogen. Cryoprotection is not necessary.<br> Ian.<br> <br> <br> <blockquote type=cite class=cite cite>Date: Wed, 24 Jan 2001 17:23:59 -0500<br> From: "Philopena, Jennifer" <jennifer.philopena@canji.com><br> Subject: cryo-preservation<br> To: "'Histonet'" <histonet@pathology.swmed.edu><br> <br> Happy Chinese New Year to all in Histoland.<br> <br> I am doing fresh frozen sections of muscle and am getting freezing artifact.<br> <br> My protocol is to remove the muscle and embed in OCT in a CO2/ methylbutane<br> slurry.  Then I section at -18, fix the sections, and b-Gal stain.  My<br> question is this: can I cryo-protect with sucrose prior to<br> embedding/freezing without fixing the muscle first?  Would the sucrose<br> interfere with staining?<br> <br> Thanks (again and again and again).<br> Jen<br> <br> <br> <br> Jennifer M. Philopena<br> Scientist 1<br> Canji, Inc.<br> 3525 John Hopkins Court<br> San Diego, CA 92121<br> jennifer.philopena@canji.com</blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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