"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
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<x-tab> </x-tab>I'm like
Anita, make a batch of fix, both LM & EM, aliquot and freeze. So far
no problem, except the Tranzer fixative for biogenic amines. This,
personal observation, has a frozen shelf life.<br>
Ian.<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Tue, 23 Jan 2001 07:17:47
-0800<br>
From: Anita Jennings <ajennings@unmc.edu><br>
Subject: Frozen paraformaldehyde<br>
To: Histonet <histonet@pathology.swmed.edu><br>
<br>
I understand the theory behind not freezing paraformaldehyde but I have
been<br>
storing aliquots at -20 in a NON frost free freezer for years (not the
same<br>
aliquot for years). According to Hogan et al "Manipulating the Mouse
Embryo"<br>
the repetitive freeze/thaw causes the solution to oxidize. But
otherwise<br>
they say go ahead and freeze. I have never seen a ppt in the fresh
thawed<br>
paraformaldehyde (4%) and don't have a problem with thorough fixation of
the<br>
tissue hmmmm. I do use phosphate buffered saline which is pH'd.
anita</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>