Davidson's Fixative


Does anyone have experience performing immunohistochemistry on Davidson's fixed
tissue?  I want to demonstrate CD31 staining in monkey eyes;  DAKO  CD31 clone
JC/70A  works well with formalin fixed tissue, but not with Davidsons in my lab.
Any suggestions would be appreciated: have tried various pretreatment methods
including pepsin, protease, proteinase K, citrate buffer, as well as no


Tom Crowell
Biogen, Inc.
Cambridge, MA

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