Cutting fixed brains at 20 microns

From:Nancy Maronto <>

We are asked to cut 20 micron sections of rat brains perfused in a 
paraformaldehyde fixative.  Can you experts out there suggest ways of doing 
this.  I know of adding sucrose to tissue and doing frozen sections. Which 
may be very difficult.  The equipment we have is a tissue tech cryostat and 
rotary microtomes.  Thanks in advance for your help.

Nancy Maronto
MPI Research

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