Cross reactivity to cell markers
|From:||Gayle Callis <firstname.lastname@example.org>|
Try Serotec veterinary antibody chart, some cross reactivity with all the
species mentioned. Also there are several publications, Serotec can help
with that on canine tissues; publication by JA Ramos-Vara and Marilyn
Biessenherz, J Vet Diag Invest 12:307-311,(2000), 63 markers with antigen
retrieval. J Histotechnology 13(4)1990, R Smith on evaluation of cross
species reactivity of human antibodies to animal modesl. Biogenex has a
species cross reactivity chart (their antibodies) call their tech support.
Pharmingen has a whole new pig scheme of things, some of these may
antihuman antibodies, worth a look.
At 02:23 PM 1/24/01 -0600, you wrote:
>Since I'm always looking to increase my IHC antibody repertoire, while
>incurring the least amount of expense when trying out human antibodies on
>non-primate mammalian species.... I'll turn to you folks....
>Does anyone have (positive staining) experience with any of the human CD
>markers on dog, cat, horse, pig, and cow tissue (formalin-fixed,
>paraffin-embedded only)? In the past, I haven't had much success in getting
>many of the CD markers to cross-react with non-human tissue (obviously, the
>problem is one of epitope recognition).
>I already use polyclonal CD3 and CD117 (c-kit), and monoclonal CD20 (BLA.36)
>and CD79a (JCB117). I'd really love to find an LCA that works, as well as
>other more specific lymphoid markers.
>Any help would be greatly appreciated.
>Univ. of Minnesota Veterinary Diag Lab
>St. Paul, MN
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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