Tim Coskran was asking about CD4 1F6 staining.  We are working 
with human lung tissue and had problems with retaining the tissue 
on the slides while doing antigen retreival.  We autoclave in the 
EDTA buffer as recommended but would loose a lot of the tissue.  
Based on a tip on the Histonet at the time, we started to coat the 
sections with nitrocellulose/Colloidin/Parlodion during the 
rehydration steps and have drastically reduced the amount of tissue 
lost.  You still may loose a little but nothing comparable to what we 
lost without the coating. The method is in John Keirnan's book (not 
sure of edition number but it had a green cover).

Mark


 
W. Mark Elliott, PhD
Research Associate
Pulmonary Research Laboratory
Room 292, St. Paul's Hospital
1081 Burrard Street
Vancouver BC Canada
V6Z 1Y6

604-806-8346
604-806-8351 (Fax)