CD4 1F6

From:"Coskran, Timothy M" <>


Does anyone have any experience with CD4 clone 1F6?  We've been using it on
formalin fixed primate tissue with some degree of success.  The problem is
that the shorter the fixation time the worse the morphology.  The
manufacturer suggests a 1mM EDTA buffer for antigen retrieval in a pressure
cooker.  Lymph nodes and spleens that are fixed for 24-48 hours are
destroyed by either the buffer the heat or the combination of the two.  Has
anyone tried a different antigen retrieval buffer or method and had success?

Tim Coskran

<< Previous Message | Next Message >>