CD117 (Teri Johnson)

Hi out there in histoland!  Is anyone using CD117, polyclonal antibody?  We
are titering it and have noticed some non-specific staining in muscle. The
spec sheet says to avoid proteolytic enzymes (so we avoided them).  It also
indicates that the antibody can be used "without the antigen retrieval,
while pretreatment by heating sometimes gives better staining."  What in the
heck is that supposed to mean?

-Teri Johnson
Histology Supervisor
Physicians Reference Laboratory
Overland Park, KS

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