We do all of our immunohistochemistry by hand.  We use
tissue culture trays with lids that are about 8-10
inches by 8-10 inches.  Then we either tap or glue 4
cut off 5mL or 10mL plastic pipetts.  2 pipetts are
about an inch apart on 2 sides of the tray.  We then
place our slides flat on the pipetts.  Make sure the
pipetts are the same size to keep the slides evenly
flat.  You can place about 16 slides in each tray, 8
on each side.  To humidify the tray we place a kimwipe
in the middle of the tray wet with our buffer. I hope
that this is descriptive enough to help you. Good
luck.


=====
Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051
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