freezing mice embryos

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From:Sally Prouty <ProutyS@physiology.uiowa.edu> (by way of Marvin Hanna)
To:histonet@histosearch.com
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Hi,
 I have a few questions about freezing mouse embryos. Do you have to fix the
embryo before you freeze it? Or can we just freeze it in Liquid Nitrogen
cooled isopentane? If you have to fix the embryo's what kind of fixative is
best to use and for how long?


Thank you,

Sally Prouty
HHMI/UIOWA
sally-prouty@uiowa.edu




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