Wrinkled Sections!

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From:jennifer.hoover@pharma.Novartis.com (by way of Marvin Hanna)
To:histonet@histosearch.com
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     Hello to All!  I know that this issue has been previously discussed but I
just wondered if any of the experts in Histoland could offer advise in
obtaining
nice, non-wrinkled paraffin mouse heart sections???  We generally fix the
hearts
for 48 hrs at 4C, then process for 5 hours before embedding.  The two paraffin
stations run 40 minutes each.  In addition to turning up the temperature of the
water bath are there any other suggestions?  Many thanks in advance as your
input is very much appreciated!


Jennifer Hoover
Novartis Pharmaceuticals




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