Rather than enter in on another hissing match about using patented kits in
particular the tyramide technology, go the Histonet Archives and read up on
what was discussed and how it can/could turn out. The original publication
author Mark Bobrow et al (he now works for NEN!) entered in on this
discussion, very enlightening since it was his method we would have liked
to make up inhouse and use, but was not done BECAUSE of patent rights.
Even if it is in the literature, beware, you may be in violation.  At the
time of this discussion, Bobrow participated in discussion, so they must be
aware of what is going on in the world.   

A department at this university found a better way to use another patented
technology (not TSA from NEN) in their project and WERE faced with
litigation and large fines if it was published.  They had to back off and
BUY the technology from the source who held the patent. After consulting
with patent office about using a published method and making it up in house
when the technology is currently patented,  we were advised to buy the TSA
kits even though chemicals, methods, etc are in J Histochem Cytochem. 

End of story here!  

  

  At 03:18 PM 1/16/01 -0500, you wrote:
>
>
>> You are required to buy the kits from New England Nuclear (TSA optimized
>> for animal work) or DAKO CSA kits for human tissues.  This is a patented
>> product and IF you make it up yourself and publish the work, you will be in
>> violation of NEN patent rights.
>
>    NEN hires people to scan the literature for possible violators? Then NEN
>goes to all labs that publish work in which tyramide amplification is used to
>be sure the product was purchased from them? Then what? Lawsuit? Jail? The
>method is published (J. Histochem. Cytochem) and all reagents are
available to
>the general public.
>
>Geoff
>--
>**********************************************
>Geoff McAuliffe, Ph.D.
>Neuroscience and Cell Biology
>Robert Wood Johnson Medical School
>675 Hoes Lane, Piscataway, NJ 08854
>voice: (732)-235-4583; fax: -4029
>mcauliff@umdnj.edu
>**********************************************
>
>
>
>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303