Sectioning Frozen Bone Marrow!

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     Hi All!  I am currently trying to section frozen marrow samples.  More
specifically, bone marrow that has been injected under the kidney capsule in
mice.  The kidney was harvested and snap frozen in liquid nitrogen cooled
isopentane.  I am using a Leica 3000M cryostat with both manual and motorized
options.  Any recommendations or suggestions for acheiving "nice" bone marrow
samples would be very much appreciated.  I am of course using a tungsten
knife and sectioning at colder temperatures (as recommended) of -30 C. Many
thanks as always for your help!


Jennifer Hoover
Novartis Pharmaceuticals

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