Re: quick question

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From:Jeffrey S Crews <> (by way of Marvin Hanna)
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I put in a vote for this too. We used to do all our IHC this way until
volume demanded a change to Coverplates. We still do it with some ABs
that don't seem to work with Coverplates. We expoxy 10ml plastic pipets
into the TC tray just like Ms Sheridan says and it's very convenient. The
10ml size gives enough height to easily pick up the slides. You can fit 8
slides down each side of the tray, the cover fits well to hold in the
humidity, the lid is transparent, they stack well in an incubator, etc.
Slide boxes are opaque, and you also can't grab the slides as easily. Of
course, after you make these, other labs in your building will steal them
and you'll have to make more...   jc

On Fri, 28 Jan 2000 07:40:06 -0800 (PST) Shelley Sheridan
<> writes:
>We do all of our immunohistochemistry by hand.  We use
>tissue culture trays with lids that are about 8-10
>inches by 8-10 inches.  Then we either tap or glue 4
>cut off 5mL or 10mL plastic pipetts.  2 pipetts are
>about an inch apart on 2 sides of the tray.  We then
>place our slides flat on the pipetts.  Make sure the
>pipetts are the same size to keep the slides evenly
>flat.  You can place about 16 slides in each tray, 8
>on each side.  To humidify the tray we place a kimwipe
>in the middle of the tray wet with our buffer. I hope
>that this is descriptive enough to help you. Good
>Shelley K. Sheridan
>Research Specialist
>Center for Neurodegenerative Disease Research
>University of Pennsylvania Medical School
>Philadelphia, PA 19104
>Do You Yahoo!?
>Talk to your friends online with Yahoo! Messenger.

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