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From:Mary Latimer <> (by way of Marvin Hanna)
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Dear David  I do whole rat brain fixation by transcardial perfusion this
tissue can be used for immunohistochemistry by frozen section within 1
hour and seems to be fine although I would not like to test it with a
parafin wax schedule.....if you want details of methods and times please
contact me direct......Mary

On Mon, 17 Jan 2000, David Grehan wrote:

> Hi
> Does anyone rapidly fix(within one hour) whole mammalian organs e.g
> Brain, heart lungs etc. using microwaves or just heating in a
> conventional oven .If so, how presentable is the tissue, can you prevent
> over cooking.
> Yours Sincerely
> David Grehan

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