Re: (no subject)

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From:Amos Brooks <atbrooks@snet.net> (by way of Marvin Hanna)
To:histonet@histosearch.com
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Hi,
    When microwave fixing tissue bear in mind that Heat not only speeds up
the chemistry of fixation but also that of autolysis. My opinion is that the
acceleration (one way of the other) is probably not worth the potential
artifacts and safety concerns.
once again
Chemistry is not a science for the impatient!
Amos Brooks

David Grehan wrote:

> Hi
> Does anyone rapidly fix(within one hour) whole mammalian organs e.g
> Brain, heart lungs etc. using microwaves or just heating in a
> conventional oven .If so, how presentable is the tissue, can you prevent
> over cooking.
>
> Yours Sincerely
> David Grehan




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