Re: msh-2 and mlh-1 antibody

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From:Phyllis Davie <> (by way of Marvin Hanna)
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We are using the following antibodies.

  MLH-1   clone 14  Zymed cat#33-7800   run @ 1:100, pretreat HIER in

  MSH-2   clone FE11     Zymed cat#33-7900   run @ 1:1000, pretreat HIER
in citrate

The MSH-2 antibody is much nicer than the MLH-1, but both work on
formalin-fixed paraffin-embedded tissues (human).

Zymed contact info:
     Zymed Laboratories Inc.
     458 Carlton Court
     South San Francisco, CA 94080
     Tel:  (650)871-4494      Fax:  (650)871-4499
     web site:

Good luck,

Phyllis Davie
PhenoPath Laboratories
Seattle, WA

>Could some one tell me a good msh-2 and mlh-1 antibody  for formalin-fixed
>paraffin embedded tissues.  What procedures and pretreatment are you
>Any suggestions would be appreciated.
>Thank you very much in advance!
>Muhammad Tahseen
>Histology Supervisor,

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