Hi,
    Many moons ago when I did this often, we used a spray fixative called Spray
Cyte by Addams Scientific to fix our cytospins. The morphology was great
and the
product was simple to use.
Amos Brooks

"Nader, Alexander" wrote:

> I would like to know how (and if) cytospin-preparations are fixed in other
> laboratories. We use Acetone/Methanol 1:1 for 5 minutes for years, but
> preparing a small lecture about problems in immunocytochemistry I came along
> a huge list of different fixation protocolls for this reason.
>
> Dr. Alexander Nader
> Path. Institut Hanuschkrankenhaus
> A 1140 Wien, Oesterreich
>
> Alexander.Nader@wgkk.sozvers.at
> privat: Alexander.Nader@univie.ac.at