Re: fighting bacteria in smears
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Fri, 11 Jan 1980, Gayle Callis wrote:
> ... dark ages, we always fixed smears for Gram stain
> using heat, flamed the back of the slide in order to
> fix the bacteria ...
As 5th-year medical students in 1964-65 we enthusiastically
fought the Good Fight, sadistically slow-cooking the
Enemy on a slide and humanely incinerating their colonies
on a platinum wire loop in the flame of a Bunsen.
Medical students these days get no such heady delights
as part of their education. Unless they pay extra in fees
to be sent to Darkest Africa for 3 weeks, they don't even
find out how to test for protein in urine by boiling it in
a test tube, adding some acetic acid and holding the tube
up in front of a dark background. I could go on & on, but
won't.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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